Document Type : Original Article


1 Department of Statistics, Faculty of Mathematical Sciences, University of Guilan, Rasht, Iran.

2 Burn and Regenerative Medicine Research center, Guilan University of Medical Sciences, Rasht

3 Department of Medical Physics, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran

4 Burn and Regenerative Medicine Research center, Guilan University of Medical Sciences, Rasht, Iran

5 Caspian Faculty of Engineering, College of Engineering, University of Tehran, Tehran, Iran


This study focuses on optimizing process of the blood coagulation powder composition with response surface methodology. For this purpose, optimizing the synthesis conditions of powder composition, the response surface methodology (RSM) was used by Box-Behnken design (BBD). The variables of this method were sodium alginate (A) and zinc oxide nanoparticles (B), prepared in combination with a fixed percentage of shell powder and measured for different reactions. results showed that according to the response surface methodology, which is the most optimal case, to introduce this method as a suitable suggestion for this kind of problem in the applications of vascular surgeries, burns, etc., and direct to future pre-clinic and clinical studies.


[1] Khoshmohabat H, Paydar S, Kazemi HM, Dalfardi B. Overview of agents used for emergency hemostasis. Trauma Mon. 2016;21(1).
[2] Rossaint R, Bouillon B, Cerny V, Coats TJ, Duranteau J, Fernández-Mondéjar E, et al. Management of bleeding following major trauma: an updated European guideline. Crit care. 2010;14:1–29.
[3] Ozhathil DK, Tay MW, Wolf SE, Branski LK. A narrative review of the history of skin grafting in burn care. Medicina (B Aires). 2021;57(4):380.
[4] Peng HT. Hemostatic agents for prehospital hemorrhage control: a narrative review. Mil Med Res. 2020;7:1–18.
[5] Duarte AP, Coelho JF, Bordado JC, Cidade MT, Gil MH. Surgical adhesives: Systematic review of the main types and development forecast. Prog Polym Sci. 2012;37(8):1031–50.
[6] Ferreira CS, AFdS C, de Abreu AP, Sartori F, Ferreira AP, Cunha Peixoto Jr K. Evaluation of nylon cable ties as an alternative method of preventive hemostasis for bovine orchiectomy. Andrology-Open Access. 2015;4(02):2–5.
[7] Cheng X, Shao Z, Li C, Yu L, Raja MA, Liu C. Isolation, characterization and evaluation of collagen from jellyfish Rhopilema esculentum Kishinouye for use in hemostatic applications. PLoS One. 2017;12(1):e0169731.
[8] Arnaud F, Tomori T, Carr W, McKeague A, Teranishi K, Prusaczyk K, et al. Exothermic reaction in zeolite hemostatic dressings: QuikClot ACS and ACS+®. Ann Biomed Eng. 2008;36:1708–13.
[9] Hajosch R, Suckfuell M, Oesser S, Ahlers M, Flechsenhar K, Schlosshauer B. A novel gelatin sponge for accelerated hemostasis. J Biomed Mater Res Part B Appl Biomater. 2010;94(2):372–9.
[10] Thomas A, Harding KG, Moore K. Alginates from wound dressings activate human macrophages to secrete tumour necrosis factor-α. Biomaterials. 2000;21(17):1797–802.
[11] Lan G, Lu B, Wang T, Wang L, Chen J, Yu K, et al. Chitosan/gelatin composite sponge is an absorbable surgical hemostatic agent. Colloids surfaces B Biointerfaces. 2015;136:1026–34.
[12] Liu X, Lin T, Gao Y, Xu Z, Huang C, Yao G, et al. Antimicrobial electrospun nanofibers of cellulose acetate and polyester urethane composite for wound dressing. J Biomed Mater Res Part B Appl Biomater. 2012;100(6):1556–65.
[13] Jiang K, Long Y-Z, Chen Z-J, Liu S-L, Huang Y-Y, Jiang X, et al. Airflow-directed in situ electrospinning of a medical glue of cyanoacrylate for rapid hemostasis in liver resection. Nanoscale. 2014;6(14):7792–8.
[14] Yang X, Liu W, Li N, Wang M, Liang B, Ullah I, et al. Design and development of polysaccharide hemostatic materials and their hemostatic mechanism. Biomater Sci. 2017;5(12):2357–68.
[15] Tomizawa Y. Clinical benefits and risk analysis of topical hemostats: a review. J Artif Organs. 2005;8:137–42.
[16] Ghezal A, Khayat KH. Optimizing self-consolidating concrete with limestone filler by using statistical factorial design methods. Mater J. 2002;99(3):264–72.
[17] Yeten B, Castellini A, Guyaguler B, Chen WH. A comparison study on experimental design and response surface methodologies. In: SPE Reservoir Simulation Symposium. OnePetro; 2005.
[18] Box GEP, Hunter JS. Multi-factor experimental designs for exploring response surfaces. Ann Math Stat. 1957;195–241.